The 26-year-old will be interviewed on "60 Minutes" by Lesley Stahl, who last spoke to the IT mogul in 2008, when the social networking empire was much smaller.
Their first interview produced a now-famous moment in geek communication when Zuckerberg awkwardly asked if Stahl was asking him a question or not.
After Zuckerberg paused and blinked a couple of times, Stahl said: "You're just staring at me," to which the billionaire replied: "Is that a question?"
In the latest interview Zuckerberg will speak about the hit film based on his life and business and what it is like being one the most influential CEOs in America.
"The Social Network," in which Zuckerberg is portrayed by Jesse Eisenberg, has generated Oscar buzz and taken home more than $180 million at the box office.
The "60 Minutes" interview will be broadcast Sunday at 7:00pm ET.